LSM 800 with Airyscan

Instrument name：

LSM 800 with Airyscan

Manufacturer：

ZEISS

Specifications：

Confocal   scan

·         Scanner: Two galvanometric scanning mirrors with ultrashort line and frame   flyback
   ·       Scanning speed: up to 8 images/sec   (Airyscan up to 1.6 images/sec) with 512 × 512 pixels at bi-direction scan
   ·       Scanning Field: 12.7 mm × 12.7 mm in   the intermediate image plane, with full pupil illumination.
   ·       Pinhole: Master pinhole with preset   size and position; can be adjusted as desired for multitracking.
   ·       Beampath: one Main beam splitter (MBS)   for 4 laser lines (405, 488, 561 and 640nm). Two patented Variable Secondary   Dichroics (VSDs) at 10 degree angle to incident beam for most effective   excitation light suppression.

·       Sample holder: universal sample holder and multiple well plate   holder

·       XY stage: Motorized XY scanning stage, for Mark & Find   function (xy) as well as Tile Scan (Mosaic Scan)

Objectives

·       Objective Plan-Apochromat 10x/0.45 M27

·       Objective Plan-Apochromat 20x/0.8 M27

·       Objective Plan-Apochromat 40x/0.95 M27

·       Objective Plan-Apochromat 63x/1.4 Oil DIC M27

Lasers：

Laser Diode : 405nm (5mW); 488nm (10mW); 561nm (10mW); 640nm   5mW)

Detectors

·       LSM 800 Confocal 2PMT-detection module and one Airyscan detector   for 63x

·       Transmitted light detector: ESID

Applications and Uses：

LSM   800 provides sensitive image acquisition leading to better flexibility in   live cell imaging and high-end image quality, well suited for various   research areas. The high sensitivity of the detectors allows detection of   weak fluorescent signals while employing low levels of laser illumination and   rapid frame rates. The system is capable of spectral imaging and unmixing,   and remove autofluorescence background from low signals in fixed brain   tissue. The acquisition software can acquire and stitch tiled images of   samples too large to fit within the field of view. It can easily be   programmed to capture images of multiple regions on a slide or multi-well   plate.

Contacts：

Jingrui Zuo

Contact number:

0512-65884616

E-mail：

zuo123@suda.edu.cn