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报告人:周悦欣 博士 (北京大学)

报告题目:Optimization and Applications of CRISPR/Cas System in Mammalian Cells

报告时间:2017119日(星期四)上午10

报告地点910-308

 

摘要:

In life science researches, deletion, insertion or modification of DNA sequence can help us find gene function and regulatory elements. In recent years, CRISPR/Cas system start to be used as a simple and high-efficiency genome editing tool. We developed a focused CRISPR/Cas-based lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis. Using knockout library screens, we successfully identified the host genes essential for the intoxication of cells by anthrax and diphtheria toxins, which were confirmed by functional validation. To improve the efficiency of producing reliable genomic modifications, we designed a method using a linear donor fragment containing a reporter system. Combined with a homologous recombination-independent knock-in strategy, we successfully enriched those cell clones that specifically carry the target gene mutations. We also developed a CRISPR/Cas9 imaging system for painting an entire chromosome in a live HeLa cell, making it possible for the first time to visualize the dynamics of a particular chromosome through the cell cycle.

 

简历:

    周悦欣,北京大学生命科学学院博士,主要研究方向为基因编辑技术的开发和应用。迄今为止,周悦欣博士已经在《Nature Nature Biotechnology Cell Research》等顶级期刊发表了论文7篇,总引用超过200多次,同时申请有多项国家专利,其中一项专利获得18届中国专利优秀奖。 同时,周博士也获得了博士研究生国家奖学金,北京大学三好学生标兵,北京大学研究生学术十杰等荣誉。

 

联系人:陈坚 教授

 

 


 

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