ACS Nano: Phosphorescence enzyme-mimics for timeresolved sensitive diagnostics and environment-adaptive specific catalytic therapeutics

Title：

Phosphorescence enzyme-mimics for timeresolved sensitive diagnostics and environment-adaptive specific catalytic therapeutics

Authors：

Mingyue Cui^1#, Lulu Qian^1#, Menglin Wu¹, Peiling Dai², Xueke Pang¹, Wenxin Xu¹, Zhixia Feng¹, Qiang Zhao², Houyu Wang¹*, Bin Song¹*, and Yao He¹*

Institutions：

¹Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM) and Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO−CIC), Soochow University, Suzhou 215123, China

²State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM) & Institute of Flexible Electronics (Future Technology), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.

Abstract：

Enzyme mimics (EMs) with intrinsic catalysis activity have attracted enormous interest in biomedicine. However, there is a lack of environmentally adaptive EMs for sensitive diagnosis and specific catalytic therapeutics in simultaneous manners. Herein, the coordination modulation strategy is designed to synthesize silicon-based phosphorescence enzyme-mimics (SiPEMs). Specifically, the atomic-level engineered Co–N₄ structure in SiPEMs enables the environment-adaptive peroxidase, oxidase, and catalase-like activities. More intriguingly, the internal Si–O networks are able to stabilize the triplet state, exhibiting long-lived phosphorescence with lifetime of 124.5 ms, suitable for millisecond-range time-resolved imaging of tumor cells and tissue in mice (with high signal-to-background ratio values of ∼60.2 for in vitro and ∼611 for in vivo). Meanwhile, the SiPEMs act as an oxidative stress amplifier, allowing the production of ·OH via cascade reactions triggered by the tumor microenvironment (∼136-fold enhancement in peroxidase catalytic efficiency); while the enzyme-mimics can scavenge the accumulation of reactive oxygen species to alleviate the oxidative damage in normal cells, they are therefore suitable for environment-adaptive catalytic treatment of cancer in specific manners. We innovate a systematic strategy to develop high-performance enzymemics, constructing a promising breakthrough for replacing traditional enzymes in cancer treatment applications.

IF：

18.027

Link：

https://doi.org/10.1021/acsnano.3c05552