报告人：Steven Lenhert副教授（佛罗里达州立大学）
报告题目：Lipid-based molecular recognition and catalysis
报告时间：2025年6月25日上午10：00
报告地点：909-B厅
报告摘要：
Molecular recognition in biology is generally attributed to polymers such as proteins that are capable of selective binding to other molecules. Lipids, as small molecules are not thought to be capable of molecular recognition. However, there is significant evidence that aggregates of small organic molecules such as micelles, bilayers or lipid droplets are also capable of carrying out selective binding or partitioning. We recently demonstrated this concept in several model systems, including oil/water two phase systems,(1-4) surface supported phospholipid multilayers,(4, 5) surface supported lipid bilayers,(5) and lipid vesicles.(4) In oil/water two phase systems, lipid-like solutes in the organic phase have been found to govern selective partitioning of small molecules and ions using charge as a driving force.(1-3) The system was found to be surprisingly selective depending on the solute concentration. Both kinetic and thermodynamic processes have been found to govern the molecular recognition.(2) We recently demonstrated an innovative biosensing mechanism based on indicator displacement from a two-phase system.(6) Furthermore, we have demonstrated bilayer enabled catalysis based on reagent partitioning into lipid bilayers.(4) This combinatorial way of thinking of lipid-based aggregates such as lipid nanoparticles, lipid membranes, or lipid droplets as information containing entities capable of molecular recognition and catalysis has broader implications in biochemistry, biosensing, and drug delivery.(7)
1. Bell, T. N.; Feng, K.; Calvin, G.; Van Winkle, D. H.; Lenhert, S., Organic Composomes as Supramolecular Aptamers. ACS Omega 2020,5 (42), 27393-27400.
2. Zhou, H.; Shiel, E.; Bell, T.; Lin, S.; Lenhert, S., Kinetic Mechanism of Surfactant-Based Molecular Recognition: Selective Permeability across an Oil–Water Interface Regulated by Supramolecular Aggregates. The Journal of Physical Chemistry B 2023.
3. Liu, S.; Wei, A.-T.; Wang, H.; Van Winkle, D.; Lenhert, S., Combinatorial mixtures of organic solutes for improved liquid/liquid extraction of ions. Soft Matter 2023,19 (36), 6903-6910.
4. Liu, S.; Kumar, K.; Bell, T.; Ramamoorthy, A.; Van Winkle, D.; Lenhert, S., Lipid-Based Catalysis Demonstrated by Bilayer-Enabled Ester Hydrolysis. Membranes 2024,14 (8), 168.
5. Lowry, T. W.; Kusi-Appiah, A. E.; Fadool, D. A.; Lenhert, S., Odor Discrimination by Lipid Membranes. Membranes 2023,13 (2), 151.
6. Tocci, V.; Shiel, E.; Zhou, H.; Liu, S.; Bell, T.; Lenhert, S., Partitioning Indicator Displacement Assay. ACS Omega in press.
7. Bell, T. N.; Kusi-Appiah, A. E.; Tocci, V.; Lyu, P.; Zhu, L.; Zhu, F.; Van Winkle, D.; Cao, H.; Singh, M. S.; Lenhert, S., Scalable Lipid Droplet Microarray Fabrication, Validation, and Screening. Plos one 2024,19 (7), e0304736.
个人简介：
Steven Lenhert is an Associate Professor in the department of Biological Science and director of the Integrative NanoScience Institute at the Florida State University (FSU). He completed his doctoral degree in Biology from the University of Muenster in 2004. He did postdoctoral research at the Karlsruhe Institute of Nanotechnology in Germany and Northwestern University in the USA. He teaches courses on introductory biology, synthetic biology, and nanotechnology. He has pioneered the use of arrays of micro- and nanoscopic lipid droplets for miniaturized high throughput screening and biosensor arrays. His current research seeks to understand how lipid-based aggregates function to encapsulate, recognize, and organize molecules in biological and synthetic environments.
联系人：迟力峰教授