题目： | Millisecond-Range Time-Resolved Bioimaging Enabled through Ultralong Aqueous Phosphorescence Probes |
作者： | Mingyue Cui 1, Peiling Dai 2, Jiali Ding 1, Manjing Li 1, Rong Sun 1, Xin Jiang 1, Menglin Wu 1, Xueke Pang 1, Mingzhu Liu 1, Qiang Zhao 2, Bin Song 1,* Yao He 1,* |
单位： | 1Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Jiangsu, Suzhou 215123 (China) 2State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM) & Institute of Flexible Electronics (Future Technology), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023 (China) |
摘要： | Probes featuring room-temperature phosphorescence (RTP) are promising tools for time-resolved imaging. Itis worth noting that the time scale of time-resolved bioimaginggenerally ranges around the microsecond level, because of the short-lived emission. Herein, the first example of millisecondrange time-resolved bioimaging is illustrated, which is enabled through a kind of ultralong aqueous phosphorescence probes (i.e., cyclo-(Arg-Gly-AspD-Tyr-Cys)-conjugated zinc-doped silica nanospheres), with a RTP emission lasting for ~5 s and a lifetime as long as 743.7 ms. We demonstrate that live cells and deep tumor tissue in mice can be specifically targeted through immune-phosphorescence imaging, with a high signal-to-background ratio (SBR) value of ~69 for in vitro imaging, and ~627 for in vivo imaging, respectively. We further show that, compared to that of fluorescence imaging, the SBR enhancement of millisecondrange time-resolved in vivo bioimaging is up to 105 times. |
影响因子： | 15.336 |
分区情况： | 一区 |
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责任编辑：郭佳