刘坚教授课题组及其合作者在Adv. Funct. Mater. 上发表论文

发布时间:2021-05-07访问量:265设置

题目:

Clearance of ESKAPE Pathogens from Blood Using Bacterially Activated Macrophage Membrane-Coated Silicon Nanowires

作者:

Sidi Liu,1, 2 Guimei Jiang,1, 2 Rui Shi,1, 2 Renfei Wu,1, 2 Xiang Xiao,1 Tianrong Yu,1, 2 Yijin Ren,2 Henny C. van der Mei,2, * Henk J. Busscher,2, * and Jian Liu1, *

单位:

1Institute of Functional Nano and Soft Materials (FUNSOM),  Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou 215123, People’s Republic of China

2Department of Biomedical Engineering, University of Groningen and University Medical Center Groningen, Antonius Deusinglaan 1, Groningen 9713 AV, The Netherlands

摘要:

Extracorporeal devices to cleanse blood from infecting bacteria are based upon bacterial capture to surfaces, but the current generation of capture devices has variable and inconclusive therapeutic efficacy. Here, a microfluidic device equipped with a Si capture surface with a highly periodic nanowired structure is designed. Nanowired Si surfaces are coated with macrophage membranes to benefit from the natural blood compatibility and ligand–receptor binding of macrophages. When macrophages are activated by uptake of Staphylococcus aureus or Escherichia coli, zeta potentials of activated macrophage membrane coatings become less negative than those of nonactivated ones, stimulating nonspecific bacterial capture. In addition, Toll-like receptors in bacterially activated membrane coatings on nanowired surfaces that are absent in nonactivated membrane coatings contribute to specific bacterial capture. These two factors, together with the maintenance of fluidity in activated membrane coatings, cause broad spectrum, high capture efficiencies of all six ESKAPE member pathogens, considered most threatening to human health. Surfaces with such broad-spectrum capture efficiencies have not been previously described, but are clinically most relevant because blood cleansing should start as soon as possible after a septic patient becomes symptomatic, when the causative bacterial strain is still unknown.

影响因子:

16.836

分区情况:

一区

链接:

https://onlinelibrary.wiley.com/doi/epdf/10.1002/adfm.202007613


责任编辑:向丹婷

返回原图
/